Table 2.
Summary of identified C. saccharolyticus secretome proteins with output from prediction server evaluation. a Total proteins predicted for secretion and containing Transmembrane domains as a function of preparation method. b List of proteins predicted to contain a signal peptide or transmembrane domain moiety. Proteins arranged by prediction features followed by protein type and sequence ID.
| a | |||||
|---|---|---|---|---|---|
| Technique | 5b | 5b-1 | 1-5b | 5b-3 | 3-5b |
| Predicted Secreted | 2 | 9 | 19 | 17 | 19 |
| Predicted Transmembrane Domain | 1 | 3 | 9 | 9 | 9 |
| Total | 3 | 12 | 28 | 26 | 28 |
| b | ||||||
|---|---|---|---|---|---|---|
| Protein Sequence ID | Phobius Signal Peptide | Phobius Trans. Domain | SignalP | TMHMM | SecretomeP | TatP |
| Predicted Secreted Protein | ||||||
| A4XIF5 Glycoside hydrolase, family 48 precursor | Yes | Yes | 1 | Yes | ||
| A4XIF7 Cellulose 1,4-beta-cellobiosidase precursor | Yes | Yes | 1 | Yes | ||
| A4XIF8 Cellulase, Cellulose 1,4-beta-cellobiosidase precursor | Yes | Yes | Yes | |||
| A4XHC3 Pullulanase, type I precursor | Yes | Yes | 1 | Yes | ||
| A4XG90 Putative, probable ISOPREN_C2_like protein | Yes | Yes | 1 | Yes | ||
| A4XHT4 Putative, probable 2-oxoacid ferredoxin/flavodoxin oxidoreductase protein | Yes | Yes | 1 | Yes | ||
| A4XLH1 Putative, probable polyamine oxidase | Yes | Yes | 1 | Yes | ||
| A4XLI2 Putative, probable polyamine oxidase | Yes | Yes | 1 | Yes | ||
| A4XM28 Putative, probable membrane protein | Yes | Yes | 1 | Yes | ||
| A4XKR7 Putative, probable peptidase M56 | Yes | Yes | ||||
| A4XM87 S-layer domain protein precursor | Yes | Yes | 1 | Yes | ||
| A4XM93 S-layer domain protein precursor | Yes | Yes | 1 | Yes | ||
| A4XGN5 Extracellular solute-binding protein, family 1 precursor | Yes | Yes | 1 | Yes | ||
| A4XMD5 Extracellular solute-binding protein, family 1 precursor | Yes | Yes | 1 | |||
| A4XJP0 Cell wall hydrolase autolysin precursor | Yes | Yes | 1 | Yes | ||
| A4XG54 Periplasmic binding protein Lacl transcriptional regulator precursor | Yes | Yes | 1 | Yes | ||
| A4XJ27 Ig domain protein, group 2 domain protein precursor | Yes | Yes | 1 | Yes | ||
| A4XJH6 Thiamine pyrophosphate enzyme domain protein TPP-binding | Yes | |||||
| A4XJH3 Pyruvate ferredoxin oxidoreductase, gamma subunit* | Yes | |||||
| Predicted Anchored to Transmembrane Domain | ||||||
| A4XIF6 Glycoside hydrolase, family 5 precursor | 1 | Yes | 1 | Yes | ||
| A4XJW4 3-oxoacyl-acyl-carrier-protein synthase II | 1 | |||||
| A4XME8 D-xylose ABC transporter, periplasmic substrate-binding protein precursor | 1 | Yes | 1 | |||
| A4XL01 2-alkenal reductase | 1 | 1 | Yes | |||
| A4XFY3 Putative, probable polyprotein | 1 | 1 | Yes | |||
| A4XIF2 Putative, probably acriflavin resistance protein B | 1 | Yes | 1 | Yes | ||
| A4XIF3 Putative, probable toxin complex protein | 1 | Yes | 1 | Yes | ||
| A4XJI8 Putative, probable zinc figure associated protein | 1 | Yes | 1 | Yes | ||
| A4XJY3 Putative, probable phosphoenolpyruvate carboxykinase | 1 | |||||
| A4XLH0 Putative, probable PKD domain-containing protein | 1 | 1 | Yes | |||
| A4XG91 Putative, probable transglutaminase domain protein | Yes | 3 | Yes | 4 | ||
*
Predicted as potential Tat signal peptide but motif absent.