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The effects of ocotillol on Dox-induced p53 activation. A549 cells were seeded into 6-well plate and treated with Dox (0.5 μM), Oco (0.5 μM), or the combination for 24 h. The cells were then lysed for immunoblotting to measure expression levels of p53 (a) or lysed for qRT-PCR assays to determine the BAX, PUMA, and PIG3 mRNA levels. Data were depicted as average ± SD values of 3 determinations. *P < 0.05, compared with control group; # P < 0.05, compared with Dox group.
