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. 2013 Jul 29;210(8):1493–1500. doi: 10.1084/jem.20130212

Figure 4.

Figure 4.

Human monoclonal antibodies specific for pH1N1 HA epitope involving aa 133 react strongly with a 1991 sH1N1 strain. HAI assays were completed using monoclonal antibodies derived from pH1N1-infected humans. HAI assays were completed using viruses possessing either wild-type pH1N1 HA (A/California/07/2009), pH1N1 with a G158E HA mutation, pH1N1 with a K133N HA mutation, or sH1N1 viruses from 1957 (A/Denver/1/1957), 1991 (A/Texas/36/1991), or 2007 (A/Brisbane/59/2007). Shown are minimum amounts of antibody required to inhibit agglutination of red blood cells in the HAI assay. Data are representative of three independent experiments.