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. 2013 Jul 29;210(8):1545–1557. doi: 10.1084/jem.20122516

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© 2013 Mansour et al.

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Figure 5. — miR-223 can partially rescue TAL1-positive cells from apoptosis induced by TAL1 knockdown. (A) Doxycycline-inducible TAL1-knockdown Jurkat S1C1 cells were retrovirally transduced to express miR-223, such that its expression was no longer under the control of TAL1. Growth kinetics were assessed with and without the addition of doxycycline, compared with control Jurkat S1C1 cells expressing a mutant miR-223 in which four bases of the seed sequence had been mutated (mut223). Values represent mean ± SD from two experiments performed in triplicate. (B) Percentage of Annexin V–positive cells as assayed by flow cytometry at 48 h after the addition of doxycycline. (C) Change in cleaved caspase-3 and -7 activity as determined by luminescence in the Caspase-Glo 3/7 assay. Values represent mean ± SEM fold changes from two independent experiments performed in triplicate. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 by two-tailed Student’s t test. (D) Western blot analysis showing equivalent TAL1 knockdown between samples.