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. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: Methods Mol Biol. 2009;579:3–18. doi: 10.1007/978-1-60761-322-0_1

Figure 3.

Figure 3

Distribution of protein markers on Western blots (A) and of gangliosides on thin-layer chromatography (B) in subcellular fractions from mouse cerebral cortex. Subcellular fractions included total homogenate (TH), crude myelin (My), Ficoll gradient purified NS mitochondria (FM), Ficoll and sucrose gradient purified non-synaptic mitochondria (NS), and Ficoll and sucrose gradient purified synaptic mitochondria (Syn). Western blots were performed to determine the distribution of specific protein markers for the inner mitochondria membrane (complex IV, subunit IV), outer mitochondrial membrane (monoamine oxidase A), myelin (proteolipid protein), synaptosomal membrane (SNAP25), cytoskeleton (β-actin), nuclear membrane (proliferating cell nuclear antigen), Golgi membrane (Tuberin), and microsomal membrane (calnexin). GM1a was visualized on TLC plates with cholera toxin b immunostaining as described in Methods. Std, is GM1a.