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. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: Gastroenterology. 2009 Sep 10;137(6):2074–2083. doi: 10.1053/j.gastro.2009.09.002

Figure 3. Mechanisms of EPEC induced Inhibition of SERT.

Figure 3

Figure 3

A)Kinetic studies of EPEC induced inhibition. A Michaelis–Menten plot from a representative experiment is shown. N=5. * P < 0.01 compared to control. B) Cell-surface expression of SERT. Caco-2 monolayers were infected with wild-type EPEC for 60 minutes and subjected to biotinylation. C) SERT expression in transiently transfected Caco-2 cells. SERT expression in wild type untransfected and cells transiently transfected with SERT-GFP construct utilizing ant-GFP antibodies. D) EPEC does not alter cell-surface expression of SERT-GFP: Representative blots from 3 different experiments are shown.