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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Exp Cell Res. 2013 May 10;319(13):2113–2123. doi: 10.1016/j.yexcr.2013.05.005

Fig. 1. RPMA technology.

Fig. 1

Sample lysates and controls are immobilized on a nitrocellulose-coated slide and probed with a primary and secondary antibody. The signal is amplified via horseradish peroxidase-mediated deposition of biotinyl tyramide. Cell lysates are prepared in a 2-fold dilution series, allowing analysis within the linear dynamic range for each sample-antibody pair.