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. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: Nat Med. 2012 Apr 15;18(5):759–765. doi: 10.1038/nm.2736

Figure 4. Mitochondrial transfer from BMSCs 24 h after LPS.

Figure 4

(a) Image of an LPS lung shows AT2 cells (green) and instilled mBMSCs (arrowheads) expressing DsRed (red). Alv, alveoli. Line, 10 µm. (b) Green, red and merged renditions of a single AT2 cell show fluorescence of surfactant protein B (SPB) (upper panel) and DsRed (middle panel). Images were obtained at the indicated levels. The cartoon depicts the optical planes in the AT2 cell. The integrated image projection along the depth (Y-Z) axis, analyzed at the cross-point of the dotted lines in merge image, shows the intracellular location of DsRed fluorescence (bracket). Line, 2 µm. Replicated in 5 lungs (20 cells). (c) Gels show amplified DNA sequences from isolated AT2 cells. hCO1, human cytochrome oxidase 1; hCO2, human cytochrome oxidase 2; hBMSC, human BMSCs; mBMSC, mouse BMSCs; HEK, human embryonic kidney cells; mGapdh: mouse glyceraldehydes 3-phosphate dehydrogenase. Repeated 4 times. (d) Group data are flow cytometry determinations. WT, wild-type mBMSCs; mutBMSC, mBMSCs expressing mutant Cx43. n = 4 lungs for each bar, * p < 0.05 versus 0 h.