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. 2013 Feb 20;33(8):3276–3283. doi: 10.1523/JNEUROSCI.0425-12.2013

Figure 3.

Figure 3.

SiRNA against Foxp2 was effective at reducing protein levels both in vivo and in vitro. A, In vivo infusion of siRNA directed against Foxp2 mRNA on the day of birth and the subsequent day significantly reduced Foxp2 protein levels 24 h later but had no impact on the closely related Foxp1 (F < 1.0, p > 0.05). N = 2–3/group with equal numbers of males and females. B, In vitro transfection of primary cell cultures on DIV 3 with siRNA directed against Foxp2 mRNA reduced Foxp2 protein levels in both the cortex and striatum. C, By postnatal day 4, we again confirmed a sex difference in Foxp2 protein levels, with males having higher levels than females, and the suppression of Foxp2 levels by siRNA was no longer apparent in males (F < 1.0, p > 0.05). However, females treated with Foxp2 siRNA showed an increase in Foxp2 protein compared with scrambled control treated females, suggesting a rebound following inhibition (F test; p < 0.05), n = 8 /group with equal numbers of males and females). Groups with the same symbol (*) are significantly different from each other.