Table 1.

Antagomir-21 Experiments Design (Different Parameters Measured)

1 day	3 days	7 days	14 days	21 days	28 days
BBB (n=4)
					LI
WB (FasL, PTEN and PDCD4) (n=4)	WB (FasL, PDCD4 and PTEN) (n=4)
qRT-PCR (miR-21) (n=4)	qRT-PCR (miR-21) (n=4)
ICH (PTEN & PDCD4), TUNEL & ISH (miR-21) (n=4)	ICH (PTEN & PDCD4), TUNEL & ISH (miR-21) (n=4)

Basso, Beattie, and Bresnahan score (BBB) for measuring hindlimb locomotor ability was repeat measured at 1, 3, 7, 14, 21, and 28 days post-injury. After the final behavioral tests, which occurred 28 days after surgery, the animals were terminally euthanized for the measure of the lesion identification.

LI: Lesion identification by Cresyl violet staining for assessing lesion size and spared tissue following behavioral analyses.

WB: Western blot analysis for quantifying the protein level of FasL, PTEN, and PDCD4.

qRT-PCR: Quantitative real-time polymerase chain reaction (miR-21) for determining the inhibitory effect of antagomir-21 on miR-21 in the spinal cord.

ICH: Immunohistochemistry staining for analyzing the expression of PTEN and PDCD4.

TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling staining for detecting apoptosis cells

ISH: In situ hybridization for determining the inhibitory effect of antagomir-21 on miR-21 in the spinal cord.