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Pulmonary M3 muscarinic receptor function is unaffected by parainfluenza infection or Pam2CSK4 (TLR2/6)/ODN2395 (TLR9) pretreatment. Acetylcholine (1–10 μg/kg, intravenous) caused bronchoconstriction, measured as increases in Ppeak, by activating airway smooth muscle M3 muscarinic receptors. Acetylcholine-induced bronchoconstriction was similar between groups (n = 5 per group). Data shown represent the means ± standard errors of the mean.
