Figure 5.

CCR7-mediated ERK phosphorylation. IL-5–primed eosinophils were incubated in CCL19 (500 ng/ml) or CCL21 (500 ng/ml). (A) Western blots demonstrate an increase in phospho-ERK1/2 (pERK) after stimulation with CCL19 and CCL21. Western blots for total ERK1/2 (ERK) are included as controls. (B) ERK1/2 phosphorylation over an extended time course was assayed by Luminex multiplex assay and normalized to glyceraldehyde 3-phosphate dehydrogenase values detected in eosinophil lysates at specified time points. Data are mean values from four separate donors and expressed as the ratio to unstimulated cells (time 0).