Figure 3.

SPLUNC1 is inhibited by LPS. LPS (5 μg) was administered intranasally to C57BL/6 mice, and the mice were killed at different time points. (A) SPLUNC1 concentrations, as measured in BALF by ELISA, are compared with total cell counts in BALF at intervals of 3, 8, 24, and 48 hours. (B) SPLUNC1 expression was assessed in RNA from lung tissue by quantitative PCR at intervals of 3, 8, 24, and 48 hours. Mean values are shown (n = 4 mice/group), and are representative of three experiments. RQ, relative quantitation.