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Regulation of SPLUNC1 by LPS. (A) SPLUNC1 was measured by ELISA in BALF from naive tlr4+/+, tlr4−/−, myd88+/+, and myd88−/− mice (n = 6 mice/group). (B) Tracheal epithelial cells from C57BL/6 mice were grown in culture and treated with LPS (100 μg) for 24 and 48 hours. Control cells (−) were untreated and cultured for 48 hours. SPLUNC1 expression was measured by quantitative PCR. Mean values are shown, and are representative of three experiments. TLR4, Toll-like receptor–4. *P < 0.05.
