Figure 6.

IFN-γ and IL-13 exert different effects on SPLUNC1 in mice. Tracheal epithelial cells were cultured with IFN-γ (10 ng/ml) or IL-13 (10 ng/ml) for 48 hours. (A) SPLUNC1 mRNA expression was measured by quantitative PCR. (B) SPLUNC1 protein secretion was measured by ELISA at 48 hours and at 10 days. (C) Airway inflammation was induced in ifnγr1^+/+ or ifnγr1^−/− mice after the transfer of Th1 cells and inhaled OVA. Naive mice served as controls. SPLUNC1 was measured in BALF by ELISA. Mean values are shown (n = 5 mice/group). In each experiment, the data are representative of three experiments. IFNγr1, interferon-γ receptor–1. *P < 0.05.