Figure 1.

Prostaglandin (PG)E2 enhances Ni-induced IL-8 release from human lung fibroblasts (HLF). (A) Cells were stimulated with 200 μM nickel (Ni) with or without PGE2 for 48 hours as described in Materials and Methods. IL-8 levels in conditioned medium were measured using ELISA. Points with different letters have means that are significantly different within the Ni-treated group; ***different from respective concentration of PGE2 in the absence of Ni (P < 0.01) as determined by two-way ANOVA with Bonferroni multiple comparison test. (B) Western blot analysis indicating expression of PGE2 receptors (PTGER)1–4 in human lung fibroblasts. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is shown as a loading control. Con, control.