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. 2013 Aug 1;24(15):2350–2361. doi: 10.1091/mbc.E12-12-0868

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© 2013 Saravanan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Autonomous and nonautonomous membrane and myosin response in naturally delaminating cells. (A) Representative apical area dynamics of one naturally delaminating cell (black curve) and a NeNe (gray curve) showing pulse dampening (gray horizontal bars denote amplitude, and black arrows denote duration) during constriction or extrusion of the delaminating cell. Black horizontal bars show A_max and A_min before cell constriction or upon recovery from dampening. (B1–B3) Membrane (ECadh GFP) and myosin (sqhGFP) dynamics in the NeNe (red arrowheads) directed toward the delaminating cell. (C1, C2) Time-lapse images of a naturally delaminating cell showing the apical (arrow) and basal (arrowhead) myosin pools. Scale bar, 10 μm. See Supplemental Figures S6 and S7 for further examples.