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. 2013 Aug 1;24(15):2378–2388. doi: 10.1091/mbc.E12-12-0860

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© 2013 Zlatic et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Analysis of clathrin light-chain and AP-3 δ colocalization in PC12 cells using high-resolution deconvolution microscopy and superresolution structured illumination microscopy. (A) High-resolution deconvolution microscopy done in the Huygens algorithm and (B) Superresolution SIM of a single PC12-cell z-plane. Insert, threefold enlargement of the boxed region. Arrows direct attention to discrete puncta showing colocalization between clathrin light chain (CLC) and AP-3 δ signals. (C) Isosurface rendering through 10 z-planes of SIM microscopy in B. Scale bar, 5 μm. Boxed region includes surface reconstruction enlarged in C1 and C2. (C1) Arrows draw attention to the same discrete puncta in A and B. Dotted lines outline area of colocalization. Scale bar, 0.5 μm. (C2) A 45° rotation of C1 around a vertical axis. See Supplemental Movie S1.