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. 2013 Aug 1;24(15):2378–2388. doi: 10.1091/mbc.E12-12-0860

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© 2013 Zlatic et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Analysis of clathrin light-chain and AP-3 δ colocalization in PC12-cell early endosomes. (A, B) High-resolution deconvolution microscopy of PC12 cells incubated in the presence of vehicle or AP20187 and triple labeled with antibodies against AP-3 δ, the mCherry tag in mCh-FKBP-CLC, and EEA1. Scale bar, 5 μm. (C–E) Probability plots of the extent of pixel overlap among marker pairs (Y axis) in cells treated with vehicle (blue traces) and AP20187 (red traces). The p values were determined using the Kolmogorov–Smirnov test. Vehicle, n = 167 endosomes from 55 cells acquired from three biological replicates. AP20187, n = 203 endosomes from 68 cells acquired from three biological replicates.