Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Aug 1;24(15):2389–2397. doi: 10.1091/mbc.E13-01-0011

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Roccisana et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 4: — Insulin does not alter the distribution of mVps45. Noninfected 3T3-L1 adipocytes were incubated with or without 100 nM insulin for 30 min and then fractionated into PM, LDM, HDM, and cytosolic fractions, and 25 μg of each fraction was separated by SDS–PAGE. Immunoblot analysis with anti-GLUT4 and anti-mVps45 is shown. The endogenous mVps45 does not appear to exhibit any marked redistribution between fractions. Analysis of three experiments of this type revealed that the membrane/cytosol distribution of mVps45 does not change in response to acute insulin treatment (values are mean ± SD of three determinations).