TABLE 1:
Quantification of syntaxin 16 and mVps45 levels.
| Syntaxin 16 | mVps45 | |
|---|---|---|
| 3T3-L1 fibroblasts (copies/cell × 103) | 116 ± 21 | 45 ± 5.8 |
| 3T3-L1 adipocytes | ||
| Total (copies/cell × 103) | 635 ± 59 | 459 ± 61 |
| Membrane (copies/cell × 103) | 635 ± 59 | 203 ± 35 |
| Cytosol (copies/cell × 103) | Not detected | 247 ± 21 |
| 3T3-L1 adipocytes (copies/mg) | 1.52 ± 0.2 × 1012 | 1.10 ± 0.3 × 1012 |
| Rat adipocytes (copies/mg) | 3.6 ± 0.5 × 1012 | 2.8 ± 0.45 × 1012 |
Levels of mVps45 and Sx16 were determined in 3T3-L1 fibroblasts, adipocytes, or rat adipocytes as described in Materials and Methods. Shown are the mean values (±SD) of at least three determinations, using at least three different preparations of lysates or membrane fractions.