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. 2013 Aug 1;24(15):2419–2430. doi: 10.1091/mbc.E13-03-0126

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© 2013 Yang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Tethering Clb2 to the spindle pole body stabilizes the spindle at the end of mitosis. (A) Schematic of optogenetic spindle pole body recruitment of Clb2, and Clb2 localization under 750- and 650-nm light (inset). (B) Combined phase and fluorescence time-course images (6-min interval) when Clb2 is not recruited to the spindle pole body (750-nm light; top) vs. when Clb2 is recruited to the spindle pole body (650-nm light; bottom). Green channel represents the spindle pole body (Spc42-GFP). (C) Clb2 recruitment to the spindle pole body results in significant spindle stabilization. This phenotype is not rescued by adding an extra untagged copy of Clb2, suggesting that the defect is mainly due to the gain of Clb2–CDK function at the SPB. The average cell size in different strains is shown as the inset.