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. 2013 Aug 1;24(15):2477–2490. doi: 10.1091/mbc.E13-01-0067

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© 2013 Teske et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — CHOP facilitates transcriptional activation of ATF5 by binding to CARE elements in the ATF5 promoter. (A) Diagram of potential CARE sequences present in the ATF5 promoter region, as determined by in silico analysis. The values −2.5 and −3.0 kb are relative to the transcriptional start site. Cells were treated with MG132 (B), thapsigargin (TG; C), or no treatment (NT), and chromatin immunoprecipitation was performed with antibodies specific for ATF4, CHOP, or nonspecific IgG as a negative control. qPCR was performed, and the enrichment relative to input DNA is shown for each of three genomic regions. The regions amplified by primer sets P1 and P2 include the possible CARE sequences, whereas the primer set designated P3 amplified a region located in exon 3 that does not contain a CARE site and serves as a negative control.