TABLE 2:
Functional classes of genes requiring CHOP for full induction in response to proteasome inhibition.
| Probe IDa | Gene name | Gene symbol | Fold-NT (WT/KO)b | Fold-MG132 (WT/KO)c |
|---|---|---|---|---|
| Transcription factor | ||||
| 1417516_at | C/EBP-homologous protein | CHOP | 2.8 | 19.5 |
| 1416967_at | SRY-box–containing gene 2 | SOX2 | 9.6 | 13.9 |
| 1418271_at | Basic helix-loop-helix family, member e22 | BHLHE22 | 2.8 | 6.6 |
| 1440275_at | Runt-related transcription factor 3 | RUNX3 | 7.7 | 4.4 |
| 1421469_a_at | Signal transducer and activator of transcription 5A | STAT5A | 3.1 | 3.4 |
| 1416981_at | Forkhead box O1 | FOXO1 | 3.7 | 2.9 |
| 1427938_at | c-Myc–binding protein | MYCBP | 1.4 | 2.2 |
| 1425927_a_at | Activating transcription factor 5 | ATF5 | 3.6 | 1.8 |
| 1418589_a_at | Myeloid leukemia factor 1 | MLF1 | 2.1 | 2.5 |
| 1427603_at | Activating transcription factor 7 interacting protein 2 | ATF7IP2 | 2.2 | 6.4 |
| Translation initiation | ||||
| 1435904_at | Eukaryotic translation initiation factor 2C, 3 | EIF2C3 | 1.9 | 1.9 |
| Apoptosis | ||||
| 1418203_at | Phorbol-12-myristate-13-acetate–induced protein 1 | NOXA | 2.5 | 3.3 |
| 1436222_at | Growth arrest specific 5 | GAS5 | 2.8 | 2.1 |
| 1435448_at | BCL2-like 11 (apoptosis facilitator) | BIM | 0.7 | 1.3 |
| Phosphatase activity | ||||
| 1429514_at | Phosphatidic acid phosphatase type 2B | PPAP2B | 33.7 | 21.6 |
| 1415834_at | Dual-specificity phosphatase 6 | DUSP6 | 0.5 | 2.3 |
| 1448325_at | Myeloid differentiation primary response gene 116 | GADD34 | 1.6 | 1.4 |
| 1455728_at | Phosphatase and tensin homologue | PTEN | 1.9 | 1.2 |
| Protein folding | ||||
| 1419625_at | Heat shock protein 1A | HSPA1A | 2.7 | 3.0 |
| 1434196_at | DnaJ (Hsp40) homologue, subfamily A, member 4 | DNAJA4 | 1.1 | 2.7 |
| 1426260_a_at | UDP glycosyltransferase 1 family, polypeptide A6 | UGT1A6 | 4.2 | 7.2 |
| 1455346_at | Mannan-binding lectin serine peptidase 1 | MASP1 | 3.3 | 3.7 |
| Cytokine signaling | ||||
| 1451798_at | Interleukin 1 receptor antagonist | IL1RN | 9.0 | 12.8 |
| 1417625_s_at | Chemokine (C-X-C motif) receptor 7 | CXCR7 | 1.9 | 5.6 |
| 1436861_at | Interleukin 7 | IL7 | 1.7 | 2.1 |
| Miscellaneous | ||||
| 1421001_a_at | Carbonic anhydrase 6 | CAR6 | 2.7 | 24.9 |
| 1436555_at | Solute carrier family 7, member 2 | SLC7A2 | 3.3 | 4.5 |
| 1434714_at | ERO1-like β (Saccharomyces cerevisiae) | ERO1LB | 1.7 | 2.4 |
| 1424048_a_at | Cytochrome b5 reductase 1 | CYB5R1 | 1.1 | 1.8 |
| 1418599_at | Collagen, type XI, alpha 1 | COL11A1 | 5.9 | 3.9 |
| 1417421_at | S100 calcium-binding protein A1 | S100A1 | 4.1 | 3.6 |
| 1443250_at | Regulator of G protein signaling 2 | RGS2 | 1.0 | 1.9 |
| Fatty acid synthesis | ||||
| 1439478_at | Acyl-CoA thioesterase 2 | ACOT2 | 2.1 | 11.5 |
| 1424097_at | ELOVL family member 7, elongation of long-chain fatty acids | ELOVL7 | 8.7 | 7.8 |
aAffymetrix Mouse 430 2.0 array, probe set identifier.
bMean fluorescence intensity fold difference between WT and CHOP−/− cells, no treatment (NT).
cMean fluorescence intensity fold difference between WT and CHOP−/− cells, MG132 (8 h).