Figure 5. γδ⁺ and CD4⁺ T cells are redundant IL-17A producing T cells recruited into the graft.

Flow cytometry and histologic analysis of fully allogeneic BALB/C tracheas grafted into C57BL/6 recipients and harvested after 8 days. A, plot representing the intracellular expression of IL-17A in recipient-derived H-2K^b+ cells. B, plot representing the membranous expression of γδ and αβ TCR in IL-17A⁺ H-2K^b+ GILs. C, plot representing the distribution of CD4⁺ and CD8⁺ T cells among αβ⁺ IL-17A⁺ GILs. D, plot representing the membranous CCR6 expression in IL-17A⁺ γδ⁺ and IL-17A⁺ γδ^- GILs. E, pathologic scores of control, combined CD4- and γδ-depleted, CD4-depleted and γδ-depleted recipients, respectively. Each FACS plot is representative of 5 organs and percentages are expressed as the mean of 5 organs. The bars represent the mean ± SEM of n = 6-15 organs in each group.