Table 4.
Real-time RT-PCR with putative unique transcripts (PUTs)
| Putative unique transcript ID | Annotation (BLASTX) | Relative gene expression by qRT-PCR (2-ΔΔ CT) | Expression difference analysis of Illumina/Solexa (Log2(CT_RPKM/WT_RPKM)) |
|---|---|---|---|
| Unigene3649_All |
CBF3 protein [Glycine max] |
2.11 ± 0.18 |
7.8564 |
| Unigene5045_All |
PREDICTED: uncharacterized protein LOC100795990 isoform 1 [Glycine max] |
2.64 ± 0.51 |
2.1173 |
| CL9479.Contig1_All |
ICE-like protein [Corylus heterophylla] |
1.90 ± 0.40 |
2.2465 |
| Unigene2612_All |
PREDICTED: probable transcription factor PosF21-like [Glycine max] |
2.70 ± 0.67 |
4.3792 |
| CL26498.Contig1_All |
Basic leucine zipper transcription factor [Medicago truncatula] |
4.30 ± 2.79 |
2.0836 |
| Unigene12211_All |
PREDICTED: microtubule-associated protein 70-5-like [Glycine max] |
2.82 ± 0.11 |
2.9144 |
| CL25117.Contig1_All |
Cold acclimation protein COR413-PM1 [Medicago truncatula] |
3.51 ± 0.32 |
2.9919 |
| CL33467.Contig1_All |
PREDICTED: sugar transporter ERD6-like 5-like [Glycine max] |
2.44 ± 0.27 |
1.9228 |
| Unigene12905_All |
PREDICTED: LOW QUALITY PROTEIN: sugar transporter ERD6-like 16-like [Glycine max] |
12.59 ± 7.89 |
10.7104 |
| CL21996.Contig1_All |
PREDICTED: cysteine proteinase RD19a-like [Glycine max] |
1.78 ± 0.10 |
1.2868 |
| Unigene28735_All |
Medicago truncatula HVA22-like protein a (MTR_4g108350) mRNA, complete cds |
2.35 ± 0.28 |
2.107 |
| Unigene5543_All |
HVA22-like protein e [Medicago truncatula] |
9.91 ± 2.45 |
6.7199 |
| Unigene6814_All |
PREDICTED: HVA22-like protein k-like [Glycine max] |
7.02 ± 0.32 |
2.8637 |
| Unigene37576_All |
PREDICTED: low-temperature-induced 65 kDa protein-like [Glycine max] |
2.78 ± 1.34 |
7.4373 |
| CL11725.Contig1_All |
PREDICTED: low-temperature-induced 65 kDa protein-like [Glycine max] |
2.16 ± 0.17 |
7.5431 |
| CL5093.Contig1_All |
Hydrophobic protein LTI6B [Medicago truncatula] |
2.12 ± 0.10 |
3.8777 |
| Unigene5480_All |
Hydrophobic protein RCI2A [Arabidopsis thaliana] |
4.32 ± 0.32 |
5.8638 |
| CL26053.Contig1_All |
PREDICTED: hydrophobic protein LTI6A-like [Glycine max] |
3.43 ± 0.26 |
1.5422 |
| Unigene37577_All |
PREDICTED: low-temperature-induced 65 kDa protein-like [Glycine max] |
3.53 ± 1.07 |
6.5686 |
| CL30168.Contig1_All | seed maturation protein [Glycine tomentella] | 7.42 ± 0.88 | 3.3724 |