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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Exp Gerontol. 2013 Jan 18;48(10):1107–1119. doi: 10.1016/j.exger.2013.01.006

Figure 3.

Figure 3

Autophagy is required for extension of life span during CR. A. CLS was measured in WT, atg1Δ, atg2Δ, atg7Δ, atg8Δ, and atg11Δ strains following growth in standard minimal medium containing 2% glucose. Cell viability in CFU/mL is expressed as the log of the percentage of the number of viable cells on day 1 and is plotted as a function of time in days. B. CLS during water wash CR following growth in 2% glucose minimal medium as described in Fig. 2. C. CLS during low glucose CR following growth in minimal medium containing 0.4% glucose. D. Combination of low glucose CR and water wash CR. Yeast strains were grown in minimal medium containing 0.4% glucose followed by water washing beginning on day 3 as described in Fig. 2. E-H. The percentages of petite colonies for panels A-D, respectively, are plotted as described in Fig. 2.