Figure 5. Transgenic lines overexpress LuWD40-1 and show pollen sterility.
(A) Semi-quantitative RT-PCR of LuWD40-1 and the apt1 control shows overexpression of LuWD40-1 in the three T2 transgenic lines compared to the untransformed Prairie Grande (PG). (B) The PG lines with viable pollen (left) and the OE lines with non-viable pollen (right) as visualized by MTT staining for pollen viability. (C) Pollen viability in PG and OE lines. Pollen viability was measured using pollen grains from three flowers per plant.