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. 2013 Jul 30;8(7):e70616. doi: 10.1371/journal.pone.0070616

Figure 4. TrkB induce EMT in EC cell lines.

Figure 4

A. Left: Mesenchymal morphology induced by over-expression of TrkB in Ishikawa cells (200×). Right: Epithelial morphology induced by suppression of TrkB in HEC-1B cells (200×). B. Messenger RNA (mRNA) levels of TrkB and EMT markers as analyzed by qRT-PCR in Ishikawa cells. β-actin was included as an internal control. (*p<0.05, **p<0.01; #, not significant). C. Protein levels of TrkB and EMT markers in Ishikawa cells as analyzed by immunoblotting (Right), and further quantified by densitometry of triplicate experiments (Left, **p<0.01; #, not significant). β-actin was included as an internal control. D. mRNA levels of TrkB and EMT markers as analyzed by qRT-PCR in HEC-1B cells (*p<0.05, **p<0.01; #, not significant). E. Protein levels of TrkB and EMT markers in HEC-1B cells as analyzed by immunoblotting (Right), and further quantified by densitometry of triplicate experiments (Left, **p<0.01; #, not significant). β-actin was included as an internal control. All experiments were carried out in triplicate and repeated at least three times.

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