Figure 1.

Effect of cordycepin and/or cisplatin on morphological change in HNSCC cell lines.

Notes: Three oral cavity cancer cell lines ([A] OC3; [B] OEC-M1; and [C] FaDu) were treated in plain medium (a), medium with DMSO (0.5%) (b), medium with 100 μM cordycepin (c), medium with 300 μM cisplatin (d), medium with 600 μM cisplatin (e), medium with 100 μM cordycepin plus 300 μM cisplatin (f), and medium with 100 μM cordycepin plus 600 μM cisplatin (g) for 24 hours. Morphological changes of cells were examined under light microscopy (bar: 0.1 mm; arrow: rounded cells). Round-up cells were readily observed and are indicated by the arrows. Experiments were performed three times with similar results.

Abbreviations: HNSCC, head and neck squamous cell carcinoma; DMSO, dimethyltetrazolium bromide.