Figure 5.

Effects of cordycepin and/or cisplatin on caspase-8, caspase-9, caspase-3, and PARP protein expressions in OC3 cells.

Notes: Cells (6 × 10⁵ cells/well for OC3) were treated with plain medium, medium with DMSO (0.5%), medium with 100 μM cordycepin, medium with 300 μM cisplatin, medium with 600 μM cisplatin medium with 100 μM cordycepin plus 300 μM cisplatin and medium with 100 μM cordycepin plus 600 μM cisplatin for 6 hours 12 hours 24 hours and 36 hours respectively. Cleavage caspase-8- (43 kDa) cleavage caspase-9- (35 kDa) cleavage caspase-3- (16 kDa) and cleavage PARP- (89 kDa) specific bands were detected by Western blot. (A) Immunoblots represent the observations from one single experiment repeated three times. The integrated optical densities of (B) cleaved caspase-8 (C) caspase-9 (D) caspase-3 and (E) PARP proteins were analyzed after normalization with β-actin (43 kDa) in each lane. Data in (B–E) represent the mean ± standard error of the mean of three separate experiments. *Statistical difference when compared to the control group (P<0.05).

Abbreviations: DMSO, dimethyltetrazolium bromide; PARP, poly adenosine diphosphate-ribose polymerase.