Figure 8.

Effects of cordycepin and/or cisplatin on the protein expression of the MAPK pathway in OC3 cells.

Notes: Cells (6 × 10⁵ cells/well for OC3) were treated with plain medium, medium with DMSO (0.5%), medium with 100 μM cordycepin, medium with 300 μM cisplatin, medium with 600 μM cisplatin, medium with 100 μM cordycepin plus 300 μM cisplatin, and medium with 100 μM cordycepin plus 600 μM cisplatin for 6 hours, 12 hours, 24 hours, and 36 hours, respectively. P-JNK- (46, 55 kDa), P-ERK- (44, 42 kDa), and P-p38- (43 kDa) specific bands were detected by Western blot. (A) Immunoblot represents the observations from one single experiment repeated three times. The integrated optical densities of (B) P-JNK, (C) P-ERK1/2, and (D) P-p38 proteins were analyzed after normalization with the total protein in each lane. Data in (B–D) represent the mean ± standard error of the mean of three separate experiments. *Statistical difference when compared to the control group (P<0.05).

Abbreviations: DMSO, dimethyltetrazolium bromide; P, phosphorylated; JNK, Jun NH2-terminal kinase; ERK, signal-regulated kinase; MAPK, mitogen-activated protein kinases.