Figure 5. The glucocorticoid-induced increase in mIPSC frequency is blocked by TRPV1 antagonists.

A. Continuous recordings of mIPSCs at a holding potential of -10 mV in the presence of the TRPV1 antagonist, 5’-iodoresiniferatoxin (5’-iRFT; 1 µM). B. The same cell after application of DEX (10 µM). A₁ and B₁ are temporally-expanded, 2 s sections from boxed areas of traces A and B (arrows). C. Cumulative frequency plots of data from the recording shown in A and B indicate no DEX-induced change in mIPSC inter-event interval (P>0.05; K–S test). D. The DEX-induced increase in mIPSC frequency was blocked with bath pre-application of 5’-iRFT (n=12; p>0.05) or capsazepine (CPZ; 10 µM; n=6; P>0.05). E. Application of DEX increased mIPSC frequency in the presence of the cannabinoid type 1 receptor antagonist, AM251 (20 µM; n=8; p<0.05).