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. 2013 Jul 30;8(7):e69064. doi: 10.1371/journal.pone.0069064

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© 2013 Jalili et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Annexin V/PI staining of human melanoma cell lines after 48 hr of treatment with NVP-LDE225, cyclopamine (each at 10 µM concentration) or DMSO. Annexin V⁺/PI⁻ are apoptotic cells. Experiments were performed 3 times with similar results. One representative experiment is shown.