Figure 6. LDE225 antitumor activity in xenotransplantation model of human melanoma.

A & B) 1×10⁶ LOX OMVI human melanoma cells suspended in PBS containing 10% FCS were injected s.c into both flanks. As tumors reach the mean volume of 48 mm³, NVP-LDE225 or cyclopamine were injected on daily basis at doses of 2, 20 or 200 µg/shot. (* p<0.05, ** p<0.01). C) 7.5×10⁵ of LOX IMVI cells were inoculated as above. Tumors with the mean volume of 18 mm³ were treated intratumorally on daily basis with NVP-LDE225 or vehicle. D) Photographs of mice after treatment with NVP-LDE225, cyclopamine or vehicle showing tumor volumes.