Table 1. Genetic attributes of EAEC strains.

Strains	Phylogenetic Group	Serotype		Virulence factors detected
			Main EAEC markers	Aggregative adherence fimbriae	Iron uptake genes	T5SS-related genes	T6SS-related genes
Isolates from Asymptomatic controls
C2/4	A	O153:H2	aggR, aap	agg3A	fyuA, irp2	pic	–
C21/2	A	O9:H10	aap, astA	–	fyuA, irp2	pet,pic,sepA	sciN/G/D
C29/2	A	O9:H10	aggR, aap, astA	–	fyuA, irp2	pet,pic,sepA	sciN/D
C33/2	A	O3:H2	aggR, aap, astA	aggA	fyuA, irp2	pet,pic,sat	sciN/D/vgr
C46/3	A	ONT:H30	aap	–	–	pic	–
C44/5	D	ONT:H23	aap, astA	–	fyuA, irp2	–	sciD
C43/1	B1	O152:H8	–	–	fyuA, irp2	pet,pic	–
C52/2	B1	ONT:H27	–	aggA	fyuA, irp2	pic	–
C55/4	B1	ONT:H10	aggR, aap	–	fyuA, irp2	pet,pic,sat	–
Isolates from clinical cases
H9/3	A	O113:H12	aggR, aap	–	fyuA, irp2	pet	–
H19/2	A	ONT:H16	aggR, aap, astA	–	fyuA, irp2	pet,pic	–
H63/2	A	ONT:H−	aggR, aap	–	fyuA, irp2	pic	–
H136/4	A	O3:H2	aggR, aap, astA	aggA	fyuA, irp2	pet,pic	sciN/G/D vgr
I49/3	A	O117:H27	aggR, aap, astA	agg3A	irp2	pet,pic,sepA	sciN/G/D vgr
H42/1	D	O112:H21	aggR, aap	–	fyuA, irp2	pet,pic	–
H80/3	D	O119:H17	–	–	fyuA, irp2	pic	sciD
H92/3	D	O86:H18	aggR, aap	aggA	fyuA, irp2	pic	sciN/G/D vgr
H102/1	D	O86:H11	aggR, aap	agg3A	fyuA, irp2	–	–
I18/2	D	O86:H11	aggR, aap	–	fyuA, irp2	–	–
I29/3	D	ONT:H11	aggR, aap	–	fyuA, irp2	pic, sepA	sciN/G/D
I34/4	D	O111:H21	aggR, aap, astA	agg3A	fyuA, irp2	pet,pic,sepA	–
H153/5	B1	ONT:H21	–	–	–	–	–
H1/4	B2	ONT: H17	aggR, aap, astA	aggA	fyuA, irp2	pet,pic,sat	sciG/D
H149/5	B2	O5:H11	astA	–	fyuA, irp2	–	–
Isolates tested in volunteer studies
17-2	A	O3:H2	aggR aap,	aggA	irp2	–	–
JM221	A	O92:H33	aggR, aap,she	aggA	–	–	–
34b	B1	ONT:H11	–	–	irp2	–	–
042	D	O44:H18	aggR, aap, astA, she	aafA	irp2	pet, pic	sciN/G/D vgr