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. 2013 Jul 30;8(7):e68478. doi: 10.1371/journal.pone.0068478

Figure 2. MCAF1 knockdown induces cell cycle arrest.

Figure 2

(A) IMR90 cells were treated with the indicated siRNAs for 48 hr and analyzed by Western blotting with anti-MCAF1 and anti-β-tubulin antibodies. The images were quantitatively assessed by densitometry. (B) Growth curves for siControl, siMCAF1-1, and siMCAF1-2 cells. (C) EdU incorporation assay in control and MCAF1 knockdown cells. **P<0.01. (D) RT-qPCR analysis of the cell cycle genes at 48 hr after siRNA treatment. **P<0.01.