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. 2013 Jul 30;8(7):e68478. doi: 10.1371/journal.pone.0068478

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© 2013 Sasai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Nuclei from control and MCAF1 knockdown IMR90 cells at 4 days after siRNA treatment were digested with MNase for the indicated times and analyzed by agarose gel electrophoresis. (B) Triton-insoluble fraction at 8 day after siRNA treatment was analyzed by SDS-page followed by CBB staining. (C) Western blot analysis of total cell lysates from the indicated cells at 8 days after siRNA treatment. The images were quantitatively assessed by densitometry.