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. 2013 Jul 30;8(7):e69858. doi: 10.1371/journal.pone.0069858

Figure 1. N3-mediated neutralization against B4 genotype of E59 and C2 genotype of 5746 viruses in vitro.

Figure 1

(A) Both E59 and 5746 were pre-incubated (m.o.i. = 0.5) with various amounts of N3 for 1 h at 37°C before adding them to 3T3-SCARB2 cells. RNA was extracted 2 h after infection, and subjected to RT-PCR to detect the expression of viral genome P1. The amounts of N3 in different lanes were Lane 1∶50 µg, Lane 2∶10 µg, Lane 3∶2 µg, Lane 4∶0.4 µg, Lane 5: uninfected 3T3-SCARB2 cells as the negative control, and Lane 6: E59 viral cDNA as the positive control were included. The same loading scheme was applied to (B) where Lane 6 was replaced by 5746 virus for the control. Expression of cytosolic GADPH as the internal control of RT-PCR was detected. (C) and (D) The bar graph represents the densitometric quantification of the band intensities of viral genome P1 and GADPH from (A) and (B), respectively. The error bar of each group generated from three independent experiments was included.