Figure 9. N3 downregulated TNF-α and IL-1β in CNS and muscle tissues of 5746-infected hSCARB2-transgenic mice.

RNA was extracted from the brain, spinal cord, and muscles of 5746-infected 7-day old hSCARB2-transgenic mice treated with N3 or isotype antibody, and antibody-untreated mice as described in Fig. 4 and were subjected to quantitative RT-PCR specific to (A) TNF-α and (B) IL-1β. The relative expression of target gene normalized with the internal control of β-actin expression from the same tissue was calculated as described in Fig. 7. Unpaired student t test with Welch correction was used for statistical analysis. (*p< = 0.05, **p< = 0.01).