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The cells were stained with 7-AAD, Alexa Fluor®488-conjugated anti-Ki67, Alexa Fluor®488-conjugated anti-phospho(Ser10)-histone H3 and APC-Cy7-conjugated anti-CD45 antibodies. An isotype control staining with Alexa Fluor®488 mouse IgG1 was performed. The leukoblasts, lymphocytes and granulocytic-lineage cells were identified by CD45/SSC gating. (representative analysis).
