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. 2013 Jul 30;8(7):e69993. doi: 10.1371/journal.pone.0069993

Figure 8. Neuron number and PER2 expression are unaffected in the SCN of Q175 mutants.

Figure 8

A. Representative images of Nissl stains at 10X magnification of coronal sections of the SCN from WT (left), Q175 Het (middle) and Hom (right) mice. 3V: 3rd ventricle. oc: optic chiasm. B. Quantification of Nissl+cells in the SCN from each genotype was determined by two independent observers blind to the experimental conditions. C. SCN expression of the circadian protein, PER2, is unaltered in Q175 mutants. Mice at 12m of age were sacrificed at ZT 2 and ZT 14, the respective trough and peak of SCN PER2 expression. Representative images of ZT 14 SCN, 40X magnification, are shown from each genotype. D. The day/night difference in PER2 expression is unaltered in Q175 mutants. Quantification of PER2+cells in the SCN was determined by 2 independent observers blind to the experimental conditions.