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. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Histol Histopathol. 2008 Apr;23(4):469–478. doi: 10.14670/hh-23.469

Figure 6.

Figure 6

Human OPN detection in kidney tissue by western blotting (A) and AgNOR staining on the same membrane (B). Kidney cell lysate was immunoprecipitated with anti human OPN peptide (75-90) antibody. Immunoprecipitated complexes and 6.25ng of hOPN control were subjected to 12% SDS-PAGE and western blot analysis was performed with anti-hOPN. The blot reveals an intense 56kDa OPN protein in kidney tissue and a 60kDa purified hOPN control. After immunoblotting, membrane stained by AgNOR exhibited a similar pattern with a 56kDa OPN band in human kidney extract and a 60kDa purified hOPN protein. Protein standards (Mr) indicate the position of the molecular weight markers.