Figure 4.

Cytotoxicity in HeLa and THP-1 cells. HeLa cells ((a)–(c)) and THP-1 cells ((d)-(e)) were infected with L. pneumophila strains Lp02 ((b) and (e)) or Twr292 ((c) and (f)) for 1 h. The infected cells were cultured in the presence of 50 μg/mL gentamicin for 1 h. The cells were washed and cultured in fresh medium. After 24 h of incubation, the condition of cells was observed using phase-contrast microscope.