Figure 2.

Binding of antibodies to the overlapping gp160 peptide library coated onto SWCNT-FET. Binding of monoclonal antibody 4E5 is shown in (a) and the binding of polyclonal antibody AHP2209 is shown in (b) and (c). The gp160 epitopes that had previously been identified with these antibodies [25, AbD Serotec Datasheet] are shown above the bargraphs. In (a), the two 20-mer sequences identified by Chen et al. [25] are shown as a single contiguous sequence. The sequences of the overlapping 15-mer peptide library used in this study to dissect the previously identified epitopes are shown to the right of the bargraphs. All underlined amino acids show the sequence identity of the previously identified epitopes to the peptide library. Nonunderlined amino acids show where the sequence of the previously identified epitopes and peptide library vary (detailed in the text). The binding of the antibodies to each peptide was performed at least in quadruplicate, and the values represent normalized impedance ± SEM.