Table 4.
Rabbit cytosolic SHMT structures compared in this study. The table shows the cofactor form and the ligand present in each subunit of the structures.
| Enzyme | Chain | Form | Ligand |
|---|---|---|---|
| Unliganded wild type | A | i.a. | PO4 2− |
| B | i.a. | PO4 2− | |
| C | i.a. | MES | |
| D | i.a. | MES | |
|
| |||
| Unliganded T254A |
A | i.a. | PO4 2− |
| B | i.a. | PO4 2− | |
| C | i.a. | PO4 2− | |
| D | i.a. | PO4 2− | |
|
| |||
| T254A + glycine | A | g.d. | Gly. |
| B | g.d. | Gly. | |
| C | g.d. | Gly. | |
| D | g.d. | Gly. | |
|
| |||
| T254A + L-serine | A | g.d. | L-Ser. |
| B | g.d. | L-Ser. | |
| C | g.d. | L-Ser. | |
| D | g.d. | L-Ser. | |
|
| |||
| Unliganded T254C |
A | i.a. | H2O |
| B | i.a. | H2O | |
| C | i.a. | H2O | |
| D | i.a. | PO4 | |
|
| |||
| T254C + glycine | A | g.d. | Gly. |
| B | g.d. | Gly. | |
|
| |||
| T254C + L-serine | A | g.d. | L-Ser. |
| B | g.d. | L-Ser. | |
| C | g.d. | L-Ser. | |
| D | g.d. | L-Ser. | |
|
| |||
| Wild type + glycine + 5-CHO-H4PteGlu3 | A | g.d. | Gly. |
| B | i.a. | — | |
| C | g.d. | Gly. | |
| D | i.a. | — | |
i.a.: internal aldimine.
g.d.: gem-diamine.
MES: 2-(N-morpholino)ethanesulfonate.
5-CHO-H4PteGlu3: triglutamic form of 5-formyltetrahydrofolate.