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. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Parasitol Int. 2011 Jul 19;61(1):94–100. doi: 10.1016/j.parint.2011.07.012

Fig. 4.

Fig. 4

SDS-PAGE of rOv CaBP and western blot analysis of the purified rOv CaBP protein and native Ov CaBP in Ov somatic extracts. In panel A, rOv CaBP protein was purified under the native condition and loaded on 12.5% SDS-PAGE. Lane 1: molecular weight marker, Lane 2: E. coli lysate, Lane 3: flow through, Lane 4–6: eluted fraction 1, 2, and 3 of rOv CaBP protein, respectively. The multimeric forms of rOv CaBP protein were observed and the sizes are shown by arrows. In panel B, western blot was performed to verify the multimer of rOv CaBP protein in the eluted fraction 3 using anti-His tag antibody. Lane 1: molecular weight marker, Lane 2: eluted fraction 3 of purified rOv CaBP protein. In panel C, western blot analysis of native Ov CaBP protein in Ov somatic extracts probed with sera from mice. Lane 1: molecular weight marker, Lane 2: Ov somatic extracts reacted with mouse preimmune serum, Lane 3: Ov somatic extracts reacted with serum from rOv CaBP protein immunized mouse.