Figure 1. Two examples of the experimental flow for the study of phenotypic equilibrium in cell-state proportions cultured on PDMS substrates.

The left experiment demonstrates how the parental cells were directly cultured onto 3 separate PDMS substrates (A₀, B₀, and C₀), which were then allowed to be cultured and expanded over a 2-day period (A_t, B_t, and C_t) before flow cytometry analysis. Alternatvely, the right experiment demonstrates the separation of the parental cells into its subpopulations. The stem cells were then specifically isolated, cultured, and analyzed as in the left experiment.