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Summary of ITC data for Ca2+ binding to RasGRP1
| N | ΔH (kcal/mol) | KD (μM) | T (°C) | |
|---|---|---|---|---|
| RasGRP1EF | 0.9 ± 0.1 | −7.5 ± 0.5 | 1.3 ± 0.3 | 20 |
| RasGRP1EF | 1.0 ± 0.1 | −5.0 ± 0.2 | 0.75 ± 0.10 | 15 |
| RasGRP1CE | 1.0 ± 0.3 | −5.9 ± 0.4 | 3.7 ± 0.3 | 15 |
| RasGRP1CE E494A | no binding | 15, 20 |
