Figure 6.

Pkc98E activation in Drosophila cultured cells upregulates the level of hyper-PO4 CREB. A, Pkc98E activation in clone 8 (cl8) cells that express Notch endogenously upregulates the level of hyper-PO4 CREB. Phorbol ester (TPA), a drug that specifically activates PKC, was used to activate Pkc98E (the only PKC expressed in Drosophila cultured cells). B, TPA treatment upregulates hyper-PO4 CREB level only in S2 cells expressing Notch (S2-Notch cells) and not in S2 cells without Notch (S2 cells). Protein extract from the same number of cells was loaded in each lane of all blots. *Background band that serves as an additional loading control.